Bowtie2 problem

[rakscalloni]

Hi!

I am trying to build an index using bowtie2 to make posterior alignment using tophat2. I've downloaded the file "Drosophila_melanogaster.BDGP5.75.dna.toplevel.fa" and runned the comand "bowtie2-build -f Drosophila_melanogaster. BDGP5.75.dna.toplevel.fa Dme1_BDGP5_75" using Terminal.
The output was:
Settings:
Output files: "BDGP5.75.dna.toplevel.fa.*.bt2"
Line rate: 6 (line is 64 bytes)
Lines per side: 1 (side is 64 bytes)
Offset rate: 4 (one in 16)
FTable chars: 10
Strings: unpacked
Max bucket size: default
Max bucket size, sqrt multiplier: default
Max bucket size, len divisor: 4
Difference-cover sample period: 1024
Endianness: little
Actual local endianness: little
Sanity checking: disabled
Assertions: disabled
Random seed: 0
Sizeofs: void*:8, int:4, long:8, size_t:8
Input files DNA, FASTA:
Drosophila_melanogaster.
Error: could not open Drosophila_melanogaster.
Total time for call to driver() for forward index: 00:00:00
Error: Encountered internal Bowtie 2 exception (#1)
Command: bowtie2-build -f Drosophila_melanogaster. BDGP5.75.dna.toplevel.fa Dme1_BDGP5_75

I've already download the .fa file again, but it's still not working. Does anybody nows how to solve this problema?

Thanks

[cariboudoug]

There's an incorrect space in your input fasta name so its looking for "Drosophila_melanogaster." rather than "Drosophila_melanogaster.BDGP5.75.dna.toplevel.fa"