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  • krunal
    Junior Member
    • May 2014
    • 4

    #1

    primer3

    dear all ,
    please tell me command line for primer3 software, as after reading manual i am not be able to run. please tell me full syntax of primer3.
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    The program name is primer3_core. There are many reason why you can not run the program. Please tell us what is the error message.

    Comment

    • krunal
      Junior Member
      • May 2014
      • 4

      #3
      Dear TiborNagy

      thanks... actually i used PrimerPro and get ma results, so need to rum primer3.

      Comment

      • GenoMax
        Senior Member
        • Feb 2008
        • 7142

        #4
        If you downloaded the primer3 code from http://primer3.sourceforge.net/, then you would run primer3 (as as tibornagy suggested) providing the right options for primer3_core (which is the main program)
        Code:
        $ ./primer3_core [-format_output] [-default_version=1|-default_version=2] [-io_version=4] [-p3_settings_file=<file_path>] [-echo_settings_file] [-strict_tags] [-output=<file_path>] [-error=<file_path>] [input_file]

        Comment

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