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  • patouch74
    Member
    • May 2014
    • 16

    #1

    IDBA-UD assembly usage

    I'm trying to use IDBA-UD with metagenomic paired end data (2files R1.fq and R2.fq) but I'm not sure about the commands:

    1/ first I merge them:
    bin/fq2fa --merge --filter R1.fq R2.fq read.fa

    2/ assembly ( I just want k=51)
    bin/idba_ud -r read.fa --mink 51 --maxk 51 -o output

    Am I doing right ?

    And I have 3 outputs:
    contig-51.fa
    contig.fa
    scaffold.fa

    which output I have to use ?

    thanks

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