I'm trying to use IDBA-UD with metagenomic paired end data (2files R1.fq and R2.fq) but I'm not sure about the commands:
1/ first I merge them:
bin/fq2fa --merge --filter R1.fq R2.fq read.fa
2/ assembly ( I just want k=51)
bin/idba_ud -r read.fa --mink 51 --maxk 51 -o output
Am I doing right ?
And I have 3 outputs:
contig-51.fa
contig.fa
scaffold.fa
which output I have to use ?
thanks
1/ first I merge them:
bin/fq2fa --merge --filter R1.fq R2.fq read.fa
2/ assembly ( I just want k=51)
bin/idba_ud -r read.fa --mink 51 --maxk 51 -o output
Am I doing right ?
And I have 3 outputs:
contig-51.fa
contig.fa
scaffold.fa
which output I have to use ?
thanks