MG-RAST KEGG mapper?

[ewilbanks]

Hi folks,

I'm using MG-RAST to analyze my 454 metagenomic data and have run into an irritating feature - wondering if anyone has a workaround! In the KEGG map view, honing in on Nitrogen Metabolism, I can see the reads (blue) and contigs (red) mapped to different EC numbers, and when I hover over it will tell me how many sequences are mapped. The issue I'm having is that I ahve no way of accessing WHICH reads these are to analyze them further. Anyone have ideas?? I can't seem to find this same feature browsing or blasting the data, so I don't understand where the kegg mapper is getting this info....


Thanks!!

[cliffbeall]

Try the following:

-Click the analysis button upper right
-Click on hierarchical classification
-select your metagenome and annotation source KO (KEGG orthology)
-ask for a table
-hit generate
-use filters or search for the function
-when you find the function or enzyme the abundance number in blue is a link that opens a new tab with the reads corresponding to that function