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  • MG-RAST KEGG mapper?

    Hi folks,

    I'm using MG-RAST to analyze my 454 metagenomic data and have run into an irritating feature - wondering if anyone has a workaround! In the KEGG map view, honing in on Nitrogen Metabolism, I can see the reads (blue) and contigs (red) mapped to different EC numbers, and when I hover over it will tell me how many sequences are mapped. The issue I'm having is that I ahve no way of accessing WHICH reads these are to analyze them further. Anyone have ideas?? I can't seem to find this same feature browsing or blasting the data, so I don't understand where the kegg mapper is getting this info....


    Thanks!!
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  • #2
    Try the following:

    -Click the analysis button upper right
    -Click on hierarchical classification
    -select your metagenome and annotation source KO (KEGG orthology)
    -ask for a table
    -hit generate
    -use filters or search for the function
    -when you find the function or enzyme the abundance number in blue is a link that opens a new tab with the reads corresponding to that function

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