Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Gorbenzer
    Member
    • Apr 2012
    • 45

    metagenomics contigs taxonomy assignment

    Hi everyone,
    i'm doing metagenomic assemblies of environmental samples rich in one species (>50% of the bacterial content is coming from a single species).

    I got a good amount of contigs using Abyss (but if anyone have good suggestions i'll try other softwares) and i'll like to taxonomically assign my contigs in order to identify the contigs related to the high abundant species and analyze his gene content.

    Can anyone suggest a good software for this purpose? I've found different tools for taxonomic assignment of single reads but not much for assembled contigs.

    Thanks to anyone that will help
  • Gorbenzer
    Member
    • Apr 2012
    • 45

    #2
    up! anyone have suggestions?

    Comment

    • maubp
      Peter (Biopython etc)
      • Jul 2009
      • 1544

      #3
      blobplots? https://github.com/blaxterlab/blobology

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Cancer Drug Resistance: The Lingering Barrier to Rising Survival
        by SEQadmin2



        Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

        There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
        Yesterday, 05:17 AM
      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
        07-01-2026, 11:43 AM
      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, Yesterday, 10:08 AM
      0 responses
      6 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-07-2026, 11:05 AM
      0 responses
      8 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-02-2026, 11:08 AM
      0 responses
      31 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-30-2026, 05:37 AM
      0 responses
      29 views
      0 reactions
      Last Post SEQadmin2  
      Working...