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  • Position problem of -b0 option on Mac OS X

    A strange error, the following command works on Linux but not on Mac OS X with bwa-0.7.9a.

    bwa aln ref.fasta -b0 se.bam > sai

    The following command works on both Linux and Mac OS X

    bwa aln -b0 ref.fasta se.bam > sai

    Any idea why this is the case? I am trying to run RNA-SeQC_v1.1.7.jar on Mac OS X, which constructs bwa command in the first pattern, and it produces the following error:

    [bwa_seq_open] fail to open file '-b0' : No such file or directory

    So for some reason, bwa aln consider -b0 to be an input file instead of a option when it's positioned after the ref.fasta. Anyway to get around this? Could it be due to any compilation problem, I simply used make on Mac OS X to compile bwa.

    Thanks!

  • #2
    Command-line options are processed by different libraries on OSX and your Linux distro. The OSX libraries are more particular about option (things that begin with a hyphen, e.g. -b0) placement.

    You'll find the same behaviour with tools like 'ls'.

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