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  • DESeq2 plotPCA combine replicates

    By default, the plotPCA function in DESeq2 plots the replicates separately. Is there a way to combine replicates so that there is only one dot per group? There does not seem a built-in option for that and it's not obvious to me by looking at the source.

  • #2
    As it performs a PCA, that wouldn't really be possible.

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    • #3
      hi id0,

      There is a collapseReplicates() function in DESeq2 if you want to combine technical replicates.

      ... and you could create a new DESeqDataSet with this just for plotting.
      Last edited by Michael Love; 06-17-2014, 09:12 AM.

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