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Hi,any one have experience of denovo asembly of solexa reads having 1x coverege by using velvet.
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It is not possible to assemble 1x coverage data, because no read will overlap any other read.Originally posted by bioenvisage View Post -
and to add to Torst, most de novo assemblers will have significant problems until you sequence to between 10X and 20X coverage at a minimum.Comment
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Some reads will be overlapping.
About one third locations have 0x coverage, one third 1x, fewer 2x, even fewer 3x ...Comment
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This is simply not enough coverage. You might be able to detect some SNPs, and assembled a few short regions, but that's it. Sorry.Originally posted by 0Gen View PostComment
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