Assembling Sanger Reads

[santhosh003]

Hi,

I am new to genomics. I have got sanger reads. They are in .ab1, phd.1, seq,,scf format.
How can I do reference assembly of these files. Is there any software or program to convert all these files into a single fastq file? or is there any assembler to take all these files as input and do the assembly? What could be the process of doing reference assembly for these reads. Any help is appreciated. Thank you so much.

[maubp]

For Sanger reads, you could try PHRED/PHRAD/consed

http://www.phrap.org/phredphrapconsed.html

[sklages]

or MIRA3

http://sourceforge.net/apps/mediawiki/mira-assembler/

both run fine in our hands .. :-)

cheers,
Sven

[skycreative]

For Sanger reads, you could try PHRED/PHRAD/consed
http://www.phrap.org/phredphrapconsed.html

agree with you , it is very effective