Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • cz0013
    Junior Member
    • Jul 2014
    • 8

    #1

    bowtie2 sam files problem

    I am having problems converting some sam files that I created using bowtie2. I created the files and when I went to convert them to bam files I was given the error message no @SQ lines in the header missing header abort. When I went to look at the files to see the header problem they were blank. All of the files except for the first one that was created had this problem. The first file however did work with out any problems. Does anyone know why this may have happened and how I could fix it.
    Thanks
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    Did you give the --no-hd parameter or something?

    Comment

    • cz0013
      Junior Member
      • Jul 2014
      • 8

      #3
      Not that I know of should I have used that parameter?

      Comment

      • dpryan
        Devon Ryan
        • Jul 2011
        • 3478

        #4
        No, but that would have caused the headerless SAM file. Just specify a header file when you convert to BAM.

        Comment

        • cz0013
          Junior Member
          • Jul 2014
          • 8

          #5
          I figured the problem out. I was running Bowtie2 in the background and for some reason it didn't like this. What I did was run Bowtie2 but before the command I put screen, so it wasn't running in the background. Then it worked fine.
          Last edited by cz0013; 08-12-2015, 09:46 AM.

          Comment

          Latest Articles

          Collapse

          • SEQadmin2
            Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
            by SEQadmin2



            Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

            Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
            ...
            10-14-2026, 09:55 AM
          • SEQadmin2
            New Genomics Technologies Take Aim at Long-Standing Limits
            by SEQadmin2


            Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

            We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
            ...
            09-28-2026, 10:25 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, 10-22-2026, 11:13 AM
          0 responses
          25 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-22-2026, 10:48 AM
          0 responses
          20 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-15-2026, 10:52 AM
          0 responses
          36 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-14-2026, 05:39 AM
          0 responses
          42 views
          0 reactions
          Last Post SEQadmin2  
          Working...