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  • vl80
    Member
    • Nov 2012
    • 34

    #1

    Unable to see BAM files from CLC on geneious or IGV

    We got BAM files from our service provider on CLC workbench. However, it is not possible to visualize them on both geneious and IGV for some reason. Importation fails in Geneious. I think the issue is with the reference genome. It seems like the FASTA file is not recognised due to some season as being the same one as used for the mapping my CLC. Any suggestions are welcome.

    I have sorted the BAM files on SAMTools and generated the bai files, still no results
    Last edited by vl80; 07-15-2014, 10:49 PM.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Probably stating the obvious here. Ask the provider for the original fastq files or the reference fasta they used to generate the BAM's.

    Or you could look in the BAM by using "samtools view -h <in.bam> > <out.sam>" to see if it just a matter of having to appropriately rename the ID's in reference fasta (e.g. chrX instead of X) you are using so they match.
    Last edited by GenoMax; 07-16-2014, 02:44 AM.

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