Too many short ORF
Hi everyone,
I have a question which is likely to be naive but I cannot answer it by myself.
I'm working with 26 mRNAs (supposed to be membrane proteins) from an insect transcriptome. I found that all mRNAs have, in all 6 reading frames, several short ORF, but they don't have any "big" significative ORF.
I tried to translate known mRNAs from the same transcriptome and they have ORFs with sense. Also random mRNAs have both, bigs ORF or the same pattern of short ORFs.
Furthermore, the 26 mRNAs are supposed to be translated to proteins and unlikely to be non-coding RNAs.
I'm not thinking about the biological sense of that, which might be very interesting indeed, but if perhaps this can be due to any error in bioinformatic processing at any step or any naive question that I am not considering.
Maybe if you have dealed with this problem before you'll have any idea. I appreciate any suggestion.
Thanks in advance.
I have a question which is likely to be naive but I cannot answer it by myself.
I'm working with 26 mRNAs (supposed to be membrane proteins) from an insect transcriptome. I found that all mRNAs have, in all 6 reading frames, several short ORF, but they don't have any "big" significative ORF.
I tried to translate known mRNAs from the same transcriptome and they have ORFs with sense. Also random mRNAs have both, bigs ORF or the same pattern of short ORFs.
Furthermore, the 26 mRNAs are supposed to be translated to proteins and unlikely to be non-coding RNAs.
I'm not thinking about the biological sense of that, which might be very interesting indeed, but if perhaps this can be due to any error in bioinformatic processing at any step or any naive question that I am not considering.
Maybe if you have dealed with this problem before you'll have any idea. I appreciate any suggestion.
Thanks in advance.