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Hello,
Is that the shotgun sequencing method produces long reads?
thank you for your reply.
Is that the shotgun sequencing method produces long reads?
thank you for your reply.
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Shotgun sequencing is a strategy that can be used with conventional or NG sequencing.
Depending on definition of "long" such reads (> 1 kb) can be produced by Pacific Biosciences and Oxford Nanopore sequencer. Illumina also has a new synthetic long read technology. -
thank you for your reply.
I want to know what is the difference between Shotgun sequencing, Sanger sequencing, PacBio sequencing, sequencing illumnia?
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Not sure if you would be able to see this: http://www.nature.com/nrg/journal/v1...l/nrg2626.html
This should be freely available: http://www.molecularecologist.com/ne...eldguide-2014/ which is a resource for this article: http://dx.doi.org/10.1111/j.1755-0998.2011.03024.xComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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