Hello,
Is that the shotgun sequencing method produces long reads?
thank you for your reply.
Is that the shotgun sequencing method produces long reads?
thank you for your reply.
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Shotgun sequencing is a strategy that can be used with conventional or NG sequencing.
Depending on definition of "long" such reads (> 1 kb) can be produced by Pacific Biosciences and Oxford Nanopore sequencer. Illumina also has a new synthetic long read technology. -
thank you for your reply.
I want to know what is the difference between Shotgun sequencing, Sanger sequencing, PacBio sequencing, sequencing illumnia?
thank youComment
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Not sure if you would be able to see this: http://www.nature.com/nrg/journal/v1...l/nrg2626.html
This should be freely available: http://www.molecularecologist.com/ne...eldguide-2014/ which is a resource for this article: http://dx.doi.org/10.1111/j.1755-0998.2011.03024.xComment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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