Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • tninja
    Junior Member
    • Apr 2010
    • 2

    #1

    anyone help me on bowtie format -> sam format!

    Hi all:

    I have some bowtie aligned reads with bowtie default .map format and I need to transform them into .sam format. I tried bowtie2sam.pl in samtools/misc directory of samtools package but to my suppriseing the coverted .sam file has no head line and thus cannot be coverted to .bam format and do pileup work further.
    Anyone have idea to covert bowtie format to sam format in a right way?

    best

    Kang Tu
    postdoc in NIH
  • tninja
    Junior Member
    • Apr 2010
    • 2

    #2
    solved

    wrote some script to do that myself...

    Comment

    • baby1885
      Member
      • Jan 2010
      • 13

      #3
      You can try 'bowtie -S e_coli reads/e_coli_10000snp.fq ec_snp.sam',the -S option will make output file of sam format.

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      26 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      20 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      36 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      42 views
      0 reactions
      Last Post SEQadmin2  
      Working...