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  • lethalfang
    Member
    • Aug 2011
    • 95

    #1

    CREST outputs: what's wrong?

    Spent many days to get all the CREST dependencies to run, and anyone knows what this means?

    Code:
    CREST.pl -f tumor.bam.cover.somatic.cover -d tumor.bam -g normal.bam --ref_genome human_g1k_v37_decoy.fasta -t human_g1k_v37_decoy.2bit --blatserver localhost --blatport 2014 -r 1
    I got a bunch of stuff like:

    Code:
    SV filter starting....
    low compexity filter
    -seq value = query.fa
    -repsiz range value = 2,8
    -repsiz values = 2 to 8
    -minrep value = 4
     INFO: Program read 167 bases 
     total bases read=167 
    Sorting 2 repeat records
    End 1st sort
    Filtering repeats
    nreps=2
    AFTER FILTERS nreps=2
     End of Sequence file!
     total bases read=0 
     End of nucleotides. Exiting!
    Type distance filter
    1       223254531       +       hs37d5  33068960        -       14      hs37d5  33068960        +       1       223254531       -       19      CTX     0       0
    Germline sclip filter
    Loaded 201 letters in 1 sequences
    Searched 1260 bases in 47 sequences
    FAILED
    SV filter starting....
    low compexity filter
    -seq value = query.fa
    -repsiz range value = 2,8
    -repsiz values = 2 to 8
    -minrep value = 4
     INFO: Program read 106 bases 
     total bases read=106 
     End of Sequence file!
     total bases read=0 
     End of nucleotides. Exiting!
    Type distance filter
    Germline sclip filter
    Loaded 201 letters in 1 sequences
    Searched 398 bases in 17 sequences
    FAILED
    tumor.bam.predSV.txt file is empty.

    What's wrong here? Any idea?
  • gaoyf
    Junior Member
    • Jun 2015
    • 1

    #2
    hello,I have met the same question. The .predSV.txt is empty. Do you have solved it? Can you help me?

    Comment

    • lethalfang
      Member
      • Aug 2011
      • 95

      #3
      Originally posted by gaoyf View Post
      hello,I have met the same question. The .predSV.txt is empty. Do you have solved it? Can you help me?
      I forgot what my original problem was.
      The .predSV.txt file is pretty small, sometimes only ~ 50 lines for the whole genome.
      Have you tried the whole genome and saw an empty file?

      Comment

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