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  • darked89
    Member
    • Jun 2009
    • 38

    #1

    spliced alignments viewers?

    I got a bunch of Illumina lanes from RNA-Seq experiment and corresponding draft genome sequence. There are few alignment programs I can use which can handle splicing:

    * Tophat [http://tophat.cbcb.umd.edu/]
    * GEM [http://gemlibrary.sourceforge.net]
    * SOAPals [http://soap.genomics.org.cn/soapals.html]

    So far I am aware of only one program able to display spliced alignments (SeqMonk from http://www.bioinformatics.bbsrc.ac.uk/projects/seqmonk/) but it does not have sequence view.

    My question: are there any other programs for next gen spliced mapping visualization? If yes, which input formats they use?
  • malcook
    Member
    • Sep 2009
    • 24

    #2
    IGV displays the spliced alignments produced by Tophat just fine.

    Comment

    • darked89
      Member
      • Jun 2009
      • 38

      #3
      Originally posted by malcook View Post
      IGV displays the spliced alignments produced by Tophat just fine.
      Thank you,now it works for me as well.

      Comment

      • dmarsh
        Junior Member
        • Jul 2008
        • 2

        #4
        Spliced alignments from TopHat

        We routinely use Tablet for this.

        Comment

        • darked89
          Member
          • Jun 2009
          • 38

          #5
          Originally posted by dmarsh View Post
          We routinely use Tablet for this.
          Thanks, works as well, albeit with a scary looking strings of Ns joining two mapped parts of one read.
          Bad thing: no gene models track from gff.

          Comment

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