Hi,
I'm looking into using the Solas R package ( http://cmb.molgen.mpg.de/2ndGenerationSequencing/Solas/ ) for analysing alternative splicing, but the tutorial is a bit too scarcely documented for my limited knowledge of R, so I was wondering if anyone has tried using the package with succesful results? In particular I'm wondering if it is possible to convert a GFF/GTF gene structure file into the gene structure used internally by Solas and how this might be done.
Additionally, the package tutorial states that the analysis is done on single genes, so doing it manually on a global scale seems a bit of dead-end, so has anyone used it for purposes other than analysing a set of few genes?
Hope there are some Solas users out there
Thomas
I'm looking into using the Solas R package ( http://cmb.molgen.mpg.de/2ndGenerationSequencing/Solas/ ) for analysing alternative splicing, but the tutorial is a bit too scarcely documented for my limited knowledge of R, so I was wondering if anyone has tried using the package with succesful results? In particular I'm wondering if it is possible to convert a GFF/GTF gene structure file into the gene structure used internally by Solas and how this might be done.
Additionally, the package tutorial states that the analysis is done on single genes, so doing it manually on a global scale seems a bit of dead-end, so has anyone used it for purposes other than analysing a set of few genes?
Hope there are some Solas users out there
Thomas
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