Hello all! I need to analyze some RNAseq data in .wig (wiggle) format, that I obtained from a paper, but I am unfamiliar with this format. I want to filter for a certian set of genes (ovary related) and then sort based on differential expression. Have you had experience with .wig files? Can anyone point me in a helpfull direction?
-
-
Information about the .wig format: https://genome.ucsc.edu/goldenPath/help/wiggle.html -
I found a post on BioStar that suggested using BEDOPS to convert to the more common BED file and proceed in analysis from there.Comment
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment