Here is a Sanger FASTQ :
@SRR034462.9 VAB_0176_20090326_2_WT_deg_10pskmusc_461_18_311_F3 length=35
TGAGGGGTGGGGGGGTTTTTTTTTTTTTTTTTTTT
+SRR034462.9 VAB_0176_20090326_2_WT_deg_10pskmusc_461_18_311_F3 length=35
'4<),/;//40-8<'%2+)'((37+0,4+2.4<0:
first i convert SOLID2Sanger ,but when i handle the sangerfastq,I don't know how to convert the fourth line that the quility line;how to convert it to decimal system.preferably be perl script;
Thanks a lot!
@SRR034462.9 VAB_0176_20090326_2_WT_deg_10pskmusc_461_18_311_F3 length=35
TGAGGGGTGGGGGGGTTTTTTTTTTTTTTTTTTTT
+SRR034462.9 VAB_0176_20090326_2_WT_deg_10pskmusc_461_18_311_F3 length=35
'4<),/;//40-8<'%2+)'((37+0,4+2.4<0:
first i convert SOLID2Sanger ,but when i handle the sangerfastq,I don't know how to convert the fourth line that the quility line;how to convert it to decimal system.preferably be perl script;
Thanks a lot!
Comment