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  • upper
    replied
    Originally posted by Zigster View Post
    Code:
    echo "'4<),/;//40-8<'%2+)'((37+0,4+2.4<0:" | 
    perl -e 'while(<STDIN>){chomp;@t=split("",$_);$qual.=((ord($_)-33)." ") for (@t);}print $qual;'
    
    6 19 27 8 11 14 26 14 14 19 15 12 23 27 6 4 17 10 8 6 7 7 18 22 10 15 11 19 10 17 13 19 27 15 25
    Zigster,
    thank you very much!I fix it use your script!
    Thanks!
    upper

    Leave a comment:


  • Zigster
    replied
    Code:
    echo "'4<),/;//40-8<'%2+)'((37+0,4+2.4<0:" | 
    perl -e 'while(<STDIN>){chomp;@t=split("",$_);$qual.=((ord($_)-33)." ") for (@t);}print $qual;'
    
    6 19 27 8 11 14 26 14 14 19 15 12 23 27 6 4 17 10 8 6 7 7 18 22 10 15 11 19 10 17 13 19 27 15 25

    Leave a comment:


  • upper
    started a topic Sanger FASTQ Quality Scores

    Sanger FASTQ Quality Scores

    Here is a Sanger FASTQ :
    @SRR034462.9 VAB_0176_20090326_2_WT_deg_10pskmusc_461_18_311_F3 length=35
    TGAGGGGTGGGGGGGTTTTTTTTTTTTTTTTTTTT
    +SRR034462.9 VAB_0176_20090326_2_WT_deg_10pskmusc_461_18_311_F3 length=35
    '4<),/;//40-8<'%2+)'((37+0,4+2.4<0:

    first i convert SOLID2Sanger ,but when i handle the sangerfastq,I don't know how to convert the fourth line that the quility line;how to convert it to decimal system.preferably be perl script;
    Thanks a lot!

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