Opposite directions in homologous proteins

[de_ma]

I am analyzing protein uncharacterized bacteria so far, which have a transmembrane domain and a coiled coil domio with low identity that other characterized protein of another bacteria phylum. In prediction programs, I observed that this protein has a Coiled coil domain in C' terminus, right in the periplasm, contrary to this, the protein that is apparently homologous has the coiled-coil in the N' terminus also targeted to the periplasm.
I can not find examples of homologous proteins which have opposite directions.

[bastianwur]

How sure are you that these are actual proteins?

Because both coils and transmembrane doamins can come as well from wrong annotations.
Especially some XXX in the genome sequence will lead to coils, and even intergenic regions will display some sort of transmembrane or periplasmic domains just be chance.

[de_ma]

Hello Dear
Yes, I am sure that this proteins are well annotated.
I attached an scheme of protein topology.

How sure are you that these are actual proteins?

Because both coils and transmembrane doamins can come as well from wrong annotations.
Especially some XXX in the genome sequence will lead to coils, and even intergenic regions will display some sort of transmembrane or periplasmic domains just be chance.

[bastianwur]

My next guess would be that in both cases either start or end are missing.

If not...good luck, seems interesting.