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  • crh
    Member
    • Dec 2009
    • 46
    #1

    Illumina reads- noise in 1st 12nt

    Hi

    We have Illumina paired end reads, and when we look at them w/ TrimGalore we see the the anomaly in the 1st 12-13 bases in the sequence content plot.
    Are these adapters, and should they be trimmed?

    thanks

    charles
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  • GenoMax
    Senior Member
    • Feb 2008
    • 7142
    #2
    No trimming should be required. This is normal observation (specially for RNAseq data). See more info here: http://seqanswers.com/forums/showthr...c+bias&page=14

    Comment

    • crh
      Member
      • Dec 2009
      • 46
      #3
      Great - thanks for quick reply

      Charles

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