Hi all,
I running TASSEL3 for analysis the GBS data like following
/.../tassel3_standalone/run_pipeline.pl -Xms1g -Xmx14g -fork1 -FastqToTagCountPlugin -i /.../fastq -k /.../GBS/GBSbarcodes.txt -e ApeKI -o /.../GBS/tagCounts -endPlugin -runfork1
Memory Settings: -Xms1g -Xmx14g
Tassel Pipeline Arguments: -fork1 -FastqToTagCountPlugin -i /.../fastq -k /.../GBSbarcodes.txt -e apeki -o /.../tagCounts -c 5 -endPlugin -runfork1
[main] INFO net.maizegenetics.pipeline.TasselPipeline - Tassel Version: 3.0.169 Date: July 24, 2014
net.maizegenetics.gbs.pipeline.FastqToTagCountPlugin
Using the following FASTQ files:
/.../fastq/42A87AAXX_7_fastq.txt
Reading FASTQ file: /.../fastq/42A87AAXX_7_fastq.txt
Enzyme: ApeKI
CTCC OWB003:42A87AAXX:7late1:A01
...
CGCCTTAT OWBRec:42A87AAXX:7late1:H06
Total barcodes found in lane:48
Total barcodes found in lane:48
But no any output.
Thanks for your help.
I running TASSEL3 for analysis the GBS data like following
/.../tassel3_standalone/run_pipeline.pl -Xms1g -Xmx14g -fork1 -FastqToTagCountPlugin -i /.../fastq -k /.../GBS/GBSbarcodes.txt -e ApeKI -o /.../GBS/tagCounts -endPlugin -runfork1
Memory Settings: -Xms1g -Xmx14g
Tassel Pipeline Arguments: -fork1 -FastqToTagCountPlugin -i /.../fastq -k /.../GBSbarcodes.txt -e apeki -o /.../tagCounts -c 5 -endPlugin -runfork1
[main] INFO net.maizegenetics.pipeline.TasselPipeline - Tassel Version: 3.0.169 Date: July 24, 2014
net.maizegenetics.gbs.pipeline.FastqToTagCountPlugin
Using the following FASTQ files:
/.../fastq/42A87AAXX_7_fastq.txt
Reading FASTQ file: /.../fastq/42A87AAXX_7_fastq.txt
Enzyme: ApeKI
CTCC OWB003:42A87AAXX:7late1:A01
...
CGCCTTAT OWBRec:42A87AAXX:7late1:H06
Total barcodes found in lane:48
Total barcodes found in lane:48
But no any output.
Thanks for your help.