I'm a beginner in RNAseq analysis (Illumina, PE reads). In FastQC, I got a failure on "Per base N content" towards the 3' end (95-99bp). I would expect quality score to drop here but the scores are above 32. Is this normal?
-
-
Can you post the plot? It is possible that a fraction (above the threshold) of the reads have N's in those positions. Those could be trimmed out. -
RNAseq fastqc - Failed "Per base N content"
Thanks GenoMax for your prompt feedback.
Here you have the plots.Comment
-
That is what I had thought (looks good otherwise). A bit of trimming should get rid of the N's. Most aligners will also soft-clip those N's if they are at the end of the reads.Comment
-
Nothing to worry about then? Thanks!Comment
-
Nope. Go ahead and trim the reads (lots of other threads on trimming programs).
Re-check FastQC afterwards and that "fail" should be gone.Comment
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM
Comment