tophat job killed by run time limit

[bbm]

I got my tophat running, and then it was killed after a few minutes.


My output file said the job was killed after reaching LSF run time limit.
Exited with exit code 140.


This is the error file:
[2014-09-12 16:32:42] Beginning TopHat run (v2.0.12)
-----------------------------------------------
[2014-09-12 16:32:42] Checking for Bowtie
Bowtie version: 2.2.1.0
[2014-09-12 16:32:42] Checking for Samtools
Samtools version: 0.1.19.0
[2014-09-12 16:32:42] Checking for Bowtie index files (genome)..
[2014-09-12 16:32:42] Checking for reference FASTA file
[2014-09-12 16:32:42] Generating SAM header for /gpfs_share/hlibyar/tophat/Am_genome
[2014-09-12 16:33:03] Preparing reads
left reads: min. length=12, max. length=100, 35244255 kept reads (1989 discarded)
Warning: short reads (<20bp) will make TopHat quite slow and take large amount of memory because they are likely to be mapped in too many places
[2014-09-12 16:49:20] Mapping left_kept_reads to genome Am_genome with Bowtie2
User defined signal 2


--

I appreciate any insights you can give!

Regards,
bbm

[dpryan]

You need to request more time from the scheduler. Tophat takes hours to run, so try requesting a day to be safe.

[Brian Bushnell]

You could also switch to something faster, such as BBMap or Star. A few minutes would probably still be way too short, depending on the input size.