Tweet
I'm working on pangenome analysis myself now and have noticed the severe lack of pan genome visualisation tools present at the moment bar simple venn diagrams! I have code that will go from assembled (either single or multi fasta) and output annotated genomes and comparative analysis using panoct with different visualisation options? Its still broken at the moment for genomes > 3mb but I will put it up when its done of people want it
?
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
Comment