Hi, everybody, I have some strand non specific single-ended RNA-seq data. Are there any options present in software like TopHat, Bowtie or CuffLinks I should pay attention to for data analysis? What is the procedure that they take to guarantee the correctness of data analysis? Do they align the strand non specific reads to both strands or only pay attention to the unstrandedness when doing quantification analysis? Thank you very much.
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
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