Offline Tools or packages (prefer R) for miRNA-seq time series counts data analysis ?
Hello all,
anyone could advise me to analyze my time series data sets of miRNA-seq ?? I have counts from HTS-seq counts after mapping. I have 4 time (T) points with 2 biological conditions (BC) and each have their replicates (r) but not equal replicates for all 2 conditions in diff time points. I dont know how to analysis and deal with this kind of data ?? I mean, sure i would like to know DE miRNAs among them in different point. But initially i would like to see some short of preliminary analysis of my data ???
May i use 2 anova test ??? but i am not sure because, anova takes means into account and this case its not true because i have gene (miRNAs) counts. is it correct ???
Also looking forward, some R packages (bioconductor or any statistical tool, but prefer R) to detect DE miRNAs gene over time course.
waiting for kind guide line.
anyone could advise me to analyze my time series data sets of miRNA-seq ?? I have counts from HTS-seq counts after mapping. I have 4 time (T) points with 2 biological conditions (BC) and each have their replicates (r) but not equal replicates for all 2 conditions in diff time points. I dont know how to analysis and deal with this kind of data ?? I mean, sure i would like to know DE miRNAs among them in different point. But initially i would like to see some short of preliminary analysis of my data ???
May i use 2 anova test ??? but i am not sure because, anova takes means into account and this case its not true because i have gene (miRNAs) counts. is it correct ???
Also looking forward, some R packages (bioconductor or any statistical tool, but prefer R) to detect DE miRNAs gene over time course.
waiting for kind guide line.