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  • SOS: Prokka --centre command and the quest for a perfect .gbk file

    Hello all, i would really appreciate some help with the situation i find myself in.
    I am trying to do snp calling and visualisation for some genomes i have assembled.
    I would love to be able to use ginger/parsnp to visualise snps and connect them back to genes but for this i need an annotated genome of the day zero strain we put into people (and then sampled, collecting bugs every few weeks)
    My supervisor has forbidden me from adding the day zero genome to public databases. So i need a well annotated strain of my bacteria (none are available for this particular strain)
    I tried RAST and the genbank files it generated didn't work with harvest or mauve or IGV or BRIG.
    I am trying PROKKA but in order to get a good result i need to use the --centre "xxx" command and specify a sequencing centre ID.

    Do any of you know what the sequencing ID for sanger is?
    Or do any of you have any idea of another way i can annotate my sequence

    Much obliged
    Thank you

  • #2
    Just pick something like the initials of your institute - or really use xxx - it will show up in some of the Prokka metadata, but shouldn't matter for the analysis you are talking about.

    Comment


    • #3
      thank you
      After this PROKKA generated a .gbk file for me
      I edited this .gbk in sublime text 2 so that all the accession and version categories had values and mauve is finally reading it.

      Comment

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