Hi, I have a local www-blast server running that I'm trying to query through Blast2GO to speed up some annotation. I've followed a couple of guides that are provided online, but I believe there may be a misconfiguration in the blast2go.properties file that is perhaps creating a problem and would apreciate some input from anyone who has achieved this before.
I've provided a screenshot with all the relevant information that shows the configuration and that the blast server is accepting blastx queries.
When configuring Blast2GO (by editing blast2go.properties), I edited 2 fields only.
- the Blast.urls field to include the url of my www-blast server
- the Blast.dbs field to include the database & server with a single space between these entries ("nr http://myserver.address/blast/blast.cgi").
Then after opening the blast dialogue in Blast2GO, I noticed the blast2go.properties file had been modified to include the Blast.blastdb field referencing the nr data base I want to query on our local server. This was automatically added by the program.
As you can see from the screengrab, I'm getting the error "Problem sending data to the NCBI BLAST Server. For input string "". Check Blast Mode: blastX, blastP, etc"
Can anyone suggest how to troubleshoot or resolve this? I'm stumped.
EDIT: I should say, if I blast against the NCBI server, it works fine, suggesting this is a config error with pointing to the local blast server.
Thanks.
EDIT: image removed
I've provided a screenshot with all the relevant information that shows the configuration and that the blast server is accepting blastx queries.
When configuring Blast2GO (by editing blast2go.properties), I edited 2 fields only.
- the Blast.urls field to include the url of my www-blast server
- the Blast.dbs field to include the database & server with a single space between these entries ("nr http://myserver.address/blast/blast.cgi").
Then after opening the blast dialogue in Blast2GO, I noticed the blast2go.properties file had been modified to include the Blast.blastdb field referencing the nr data base I want to query on our local server. This was automatically added by the program.
As you can see from the screengrab, I'm getting the error "Problem sending data to the NCBI BLAST Server. For input string "". Check Blast Mode: blastX, blastP, etc"
Can anyone suggest how to troubleshoot or resolve this? I'm stumped.
EDIT: I should say, if I blast against the NCBI server, it works fine, suggesting this is a config error with pointing to the local blast server.
Thanks.
EDIT: image removed
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