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  • hometeacher
    Junior Member
    • May 2010
    • 1

    #1

    bowtie for 454 reads

    Hello,

    Does anybody have experience to use bowtie to map 454 RNA reads to genome?

    how to choose parameters?

    Thanks!


    Don
  • Torst
    Senior Member
    • Apr 2008
    • 275

    #2
    Originally posted by hometeacher View Post
    Hello, Does anybody have experience to use bowtie to map 454 RNA reads to genome? how to choose parameters?
    The 454 reads may be a bit long for Bowtie. You would be better off using the gsMapper software that Roche provides for 454 read mapping. If you don't have it, you can write to Roche and request it.

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