Tweet
Hello,
Does anybody have experience to use bowtie to map 454 RNA reads to genome?
how to choose parameters?
Thanks!
Don
Does anybody have experience to use bowtie to map 454 RNA reads to genome?
how to choose parameters?
Thanks!
Don
-
-
The 454 reads may be a bit long for Bowtie. You would be better off using the gsMapper software that Roche provides for 454 read mapping. If you don't have it, you can write to Roche and request it.Originally posted by hometeacher View Post
-
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...Yesterday, 05:17 AM -
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
Comment