Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • horvathdp
    Member
    • Dec 2011
    • 66

    #1

    cutoff for significant TMP values

    So what are people using these days as a cutoff for significance with EBseq normalized results? I am using FDR<0.05 and at least 10 TPM for all biological replicates in at least one of my treatment groups. I went that high because I noticed that I had a fair number of non-plant contigs that showed up as significantly differential when I just used the FDR cutoff, and when I required a moderate level of expression, about 90% of those non-plant contigs were removed. I'd like to know what others are using and if there is any reasons underlying the common cutoff criteria.

    Thanks!

Latest Articles

Collapse

  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM
  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 07:59 AM
0 responses
15 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
32 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
0 responses
46 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
171 views
0 reactions
Last Post SEQadmin2  
Working...