We are generating a set of ChIP-seq data and are interesting in its behavior in repetitive regions. But the mapping is a problem.
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Repetitive regions are always going to be a problem for read mapping - whether it's ChIP-Seq, RNA-Seq, or gDNA-Seq. Unless your read length (or span for paired end reads) is longer than the repeat length, there is not too much you can do.Originally posted by zhenshao View Post
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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