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  • cliff
    Member
    • Oct 2009
    • 41

    #1

    sinlge-end mapping problem

    Hi, All

    I have a paired-end dataset by illumina GA. I did two types of mapping using this dataset and BWA:

    1: Just use one read to do single-end mapping
    2: I merged read1 and read2, and then still do single-end mapping.

    Then I used SAMtools to call SNPs. Surprisingly, I found one SNP showing up by using both of mapping but the number of reads mapped to this SNP is the same...

    I am confused by this result.. should Type 2 mapping give me more reads mapped to this SNP?

    Can anybody help me on this?

    Thanks very much!

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