HI,
In my RNAseq raw data, I filtered out adapter sequence using fastx and later removed rRNA reads by mapping to rRNA reference file. When I tried to convert unaligned reads from rRNA mapping to bam format, It throws me an error that
[bam_header_read] EOF marker is absent. The input is probably truncated.
[bam_header_read] invalid BAM binary header (this is not a BAM file).
[main_samview] fail to read the header from xxx.sam
I don’t what is the problem and how to tackle it.
Any Ideas or suggestions?
In my RNAseq raw data, I filtered out adapter sequence using fastx and later removed rRNA reads by mapping to rRNA reference file. When I tried to convert unaligned reads from rRNA mapping to bam format, It throws me an error that
[bam_header_read] EOF marker is absent. The input is probably truncated.
[bam_header_read] invalid BAM binary header (this is not a BAM file).
[main_samview] fail to read the header from xxx.sam
I don’t what is the problem and how to tackle it.
Any Ideas or suggestions?
Comment