Warning Message - Blast

[vishwesh]

Hi,
When I run blast I get the following warning message. I get this when running transcripts from denovo assembly.

Warning: (1431.1) CFastaReader: Title is very long: 1081 characters (max is 1000), at line 2722851
Warning: (1431.1) CFastaReader: Title is very long: 1427 characters (max is 1000), at line 2722902
Warning: (1431.1) CFastaReader: Title is very long: 1440 characters (max is 1000), at line 2722972
Warning: (1431.1) CFastaReader: Title is very long: 1456 characters (max is 1000), at line 2723042
Warning: (1431.1) CFastaReader: Title is very long: 1065 characters (max is 1000), at line 2723114
Warning: (1431.1) CFastaReader: Title is very long: 1227 characters (max is 1000), at line 2723246
Warning: (1431.1) CFastaReader: Title is very long: 1180 characters (max is 1000), at line 2723700
Warning: (1431.1) CFastaReader: Title is very long: 1816 characters (max is 1000), at line 2723859
Warning: (1431.1) CFastaReader: Title is very long: 1708 characters (max is 1000), at line 2723931
Warning: (1431.1) CFastaReader: Title is very long: 1001 characters (max is 1000), at line 2723999
Warning: (1431.1) CFastaReader: Title is very long: 1109 characters (max is 1000), at line 2724038

How to solve this...

Regards
Vishwesh

[rhinoceros]

Shorten your headers?

[vishwesh]

Does this warning message create any problem in alignment??

[rhinoceros]

Does this warning message create any problem in alignment??

I assume it only displays the first 1k characters for the headers and that's all, but who knows. Could be a problem if your headers become non-unique because of trimming from the end. Or maybe it altogether skips those seqs? I don't know. The smart thing to do would be to shorten your headers..

[maubp]

Which version of BLAST are you using? There were some warnings added by mistake in BLAST+ 2.2.29, fixed in 2.2.30, see: http://blastedbio.blogspot.co.uk/201...modifiers.html