blast WARNING: Failed to access string index ISAM Error code is -12

[Zhan yueping]

Hi everyone,

when I try to run blastall，I got a warning like this: WARNING: Failed to access string index ISAM Error code is -12. and my code: blastall -p blastn -e 1e-3 -F F -m 8 -a 16 -d SRR1174221_read_db/seq_db -i SRR1174221_CUFF.16480.1.fasta -o SRR1174221_CUFF.16480.1.blastm8.

I run formatdb succefully.

Any ideas welcome.

Thanks,
Zhan

[Zhan yueping]

ok, I now got it!

[GenoMax]

You may want to add actual explanation/workaround to this post (rather than just saying the problem is solved) so it becomes useful to someone who finds this thread by searching for a similar error.

[Zhan yueping]

GenoMax, you are right. In fact, I didn't fixed it .But, I just change the input file in formatdb code.because the first input file has too many short reads.maybe this is problem.