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  • BioFalcon
    Junior Member
    • Dec 2014
    • 1

    TargetScan Input Files

    Hello all,

    I am new in the scheme of miRNA and I recently came across a very promising script called TargetScan. However, it seems that there is a lack of documentation regarding the input, since I am having trouble with the second input file, since I don't know if I can use sequences that don't have much in common (3' UTR from a complete transcriptome) or if they have to be in some way similar.

    Thanks in advance.
    Last edited by BioFalcon; 12-15-2014, 01:30 PM.

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
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