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Dear All,
I'm new in bioinformatics and RNA-seq tech.
In our lab we have some RNA-seq samples and we need to know if we reached a good sample saturation.
My question is, how do I extract the information from a bam/sam file and plot it as a saturation plot? I know that there are some good program to do that, but I really want to do it by myself, and straight forward my analysis pipeline
Another silly and dumb question is how do I can identify the gene (as name) from a sam file? I'm trying to understand how the alignment tools achieve that hehe
If there are some good scripts is welcome
Thanks in advance
I'm new in bioinformatics and RNA-seq tech.
In our lab we have some RNA-seq samples and we need to know if we reached a good sample saturation.
My question is, how do I extract the information from a bam/sam file and plot it as a saturation plot? I know that there are some good program to do that, but I really want to do it by myself, and straight forward my analysis pipeline
Another silly and dumb question is how do I can identify the gene (as name) from a sam file? I'm trying to understand how the alignment tools achieve that hehe
If there are some good scripts is welcome
Thanks in advance