Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Bukowski
    replied
    No, you need to ignore them just as you would for all variant-calling applications. If they're duplicates then they're PCR or optical duplicates and potential sources of bias. To eliminate the bias remove them or mark them for downstream variant calling applications. About the only time this isn't done routinely is for RNA-Seq expression studies.

    Leave a comment:


  • LiLin
    started a topic Duplicated Reads for Somatic Mutation Detection

    Duplicated Reads for Somatic Mutation Detection

    Hello, everyone,

    There is one question about somatic mutation detection, is there anyone could help me?

    Duplicated reads may be marked by Picard in bam-files, and most software don't care this tag. When we calculate CNVs, we shouldn't use duplicated reads, How about Somatic SNVs or InDels?

Latest Articles

Collapse

  • seqadmin
    Exploring Human Diversity Through Large-Scale Omics
    by seqadmin


    In 2003, researchers from the Human Genome Project (HGP) announced the most comprehensive genome to date1. Although the genome wasn’t fully completed until nearly 20 years later2, numerous large-scale projects, such as the International HapMap Project and 1000 Genomes Project, continued the HGP's work, capturing extensive variation and genomic diversity within humans. Recently, newer initiatives have significantly increased in scale and expanded beyond genomics, offering a more detailed...
    Today, 06:43 AM
  • seqadmin
    Best Practices for Single-Cell Sequencing Analysis
    by seqadmin



    While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
    06-06-2024, 07:15 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 06-21-2024, 07:49 AM
0 responses
224 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-20-2024, 07:23 AM
0 responses
231 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-17-2024, 06:54 AM
0 responses
231 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-14-2024, 07:24 AM
0 responses
28 views
0 likes
Last Post seqadmin  
Working...
X