Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Can Trimmomatic handle Colorspace Reads

    I have a RNASeq fastq file (first 4 lines are):
    =====
    @SRR179591.1.1 mendel_20110320_FRAG_BC_Ryan_RNA_Seq_2_58_235_F3 length=50
    T.33.2010.0313.1231222320.1.3312.0.12.0212.0.013000
    +SRR179591.1.1 mendel_20110320_FRAG_BC_Ryan_RNA_Seq_2_58_235_F3 length=50
    !!A+!@>A5!@59%!=A'6=4@5'A!9!55=1!(!0-!'%-%!&!%&%60'
    =====

    This fastq format is Color-space. I am wondering if Trimmomatic can handle such file by trimming reads based on quality and adapters?
    (I did not see in the documentation of Trimmomatic that it can handle Color-space reads).

  • #2
    You will need to use Cutadapt: https://code.google.com/p/cutadapt/

    Comment


    • #3
      Thank @GenoMax.
      Actually, I have 100 fastq files. 95 of them contain quality values encoded in Phred33 (so I processed them with Trimmomatic), while 5 files are Color-space. I wanted to use Trimmomatic for all the 100 files for simplicity but it seems that I need to process those 5 files with Cutadapt.

      Comment


      • #4
        If you are worried about consistency then you could re-process them all with cutadapt.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Best Practices for Single-Cell Sequencing Analysis
          by seqadmin



          While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
          06-06-2024, 07:15 AM
        • seqadmin
          Latest Developments in Precision Medicine
          by seqadmin



          Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

          Somatic Genomics
          “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
          05-24-2024, 01:16 PM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Yesterday, 06:58 AM
        0 responses
        13 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 06-06-2024, 08:18 AM
        0 responses
        20 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 06-06-2024, 08:04 AM
        0 responses
        18 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 06-03-2024, 06:55 AM
        0 responses
        13 views
        0 likes
        Last Post seqadmin  
        Working...
        X