Benchmark - Comparison of Different NGS Mappers

[ecSeq Bioinformatics]

In order to compare read aligner, we use published real-life paired-end DNA/RNA-Seq dataset. All optimal alignments (also multiple mapping loci) of 100,000 read pairs of each sample were obtained by RazerS 3 (full sensitivity mapping tool). In the benchmark, we measured the performance in finding all optimal hits of different NGS mappers with default parameters. We use reads with up to 10 multiple mapping loci, allowing up to 10 errors (mismatches and indels) as 'true positives' in this benchmark.



Visit our benchmark page for more information.

There is also a lively discussion on biostars.