Hi there,
I have a problem with 454 paired-end in a re-sequencing data set.
Newbler tell me that the paired end are in opposite strand (-> <- or <- ->) but when I try to allign (with blastn) the PE reads against the reference I always find that the two arms are on the same orientation (-> -> or <- <-).
Has someone noted the same problem ?
Is this a bug ?
Thanks in advance
Alessandro
I have a problem with 454 paired-end in a re-sequencing data set.
Newbler tell me that the paired end are in opposite strand (-> <- or <- ->) but when I try to allign (with blastn) the PE reads against the reference I always find that the two arms are on the same orientation (-> -> or <- <-).
Has someone noted the same problem ?
Is this a bug ?
Thanks in advance
Alessandro
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