Hi,
I was wondering whether DESeq2 has a feature to correct for gene-length and GC-content bias simultaneously?
I currently am correcting for GC-content bias using the method explained in DESeq2 manual (i.e., the normalizationFactors matrix). But I need to do correction for gene-length, since I'm trying to find differentially expressed genes between three species. Assuming that L_ij is the length of gene i in condition (subgroup) j, how do I incorporate matrix L along with the normalizationFactors matrix into the data set?
I'd appreciate any help.
Thanks,
Golsheed
I was wondering whether DESeq2 has a feature to correct for gene-length and GC-content bias simultaneously?
I currently am correcting for GC-content bias using the method explained in DESeq2 manual (i.e., the normalizationFactors matrix). But I need to do correction for gene-length, since I'm trying to find differentially expressed genes between three species. Assuming that L_ij is the length of gene i in condition (subgroup) j, how do I incorporate matrix L along with the normalizationFactors matrix into the data set?
I'd appreciate any help.
Thanks,
Golsheed
Comment