I am trying to use cuffnorm after tophat and cuffquant. I am using the gencodevM3 annotation and have filtered it so that it only contains CDS and exon information. However, when I run cuffnorm on my samples it only writes information to the genes and isoform tables and leaves the CDS and tss tables empty. Does anyone have any ideas? Thanks
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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