Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Merging different reference genomes

    Hi to everyone,
    i'm trying to replicate a pipeline from an article in which they says that they had merged different information from different reference genomes to build an unique and complete reference genome.
    The article is by Billy Li and here is the link



    and what they said is : " we mapped RNA-seq
    reads not only to the reference genome or to the transcriptome
    but to a combination of the hg19 reference genome plus exonic
    sequences surrounding all currently known splicing junctions
    from gene models available in annotation from Gencode, RefSeq,
    Ensembl and UCSC Genes"

    So here is my problem, i don't understand how they performed this task.
    I tried in different ways to merge the reference genomes,
    i tried merging the genome.fa file from Wholegenome folder from my reference files,
    i tried to merge the chromosome files together and after fuse them in a unique file,
    i also tried to merge the genome.fa from the BWAIndex folder that is already present in to the reference folders, but nothing works.
    I index the merged files but the program doesn't recognize the index.
    does anyone have and idea about how to perform this merging?

    thanks all

  • #2
    Honestly, it would make more sense to just use a split-read mapper like STAR, tophat, or segemehl. You can then just map to the genome and be done with that step instead of needing to play around with adding splice junction sequences in.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Latest Developments in Precision Medicine
      by seqadmin



      Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

      Somatic Genomics
      “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
      05-24-2024, 01:16 PM
    • seqadmin
      Recent Advances in Sequencing Analysis Tools
      by seqadmin


      The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
      05-06-2024, 07:48 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 06:55 AM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-30-2024, 03:16 PM
    0 responses
    23 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-29-2024, 01:32 PM
    0 responses
    27 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-24-2024, 07:15 AM
    0 responses
    214 views
    0 likes
    Last Post seqadmin  
    Working...
    X