Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • atape
    started a topic Abnormal reading of FASTA file by PAL2NAL

    Abnormal reading of FASTA file by PAL2NAL

    I am making a script that produces as an output a protein alignment in fasta format and an appropriate mRNA fasta file for latter use with the PAL2NAL algorithm that gives a codon aware alignment.

    PAL2NAL is reading the mRNA fasta file normally, the same cannot be said for the protein file. This is the error that the web based interface gives:

    ERROR: number of input seqs differ (aa: 82; nuc: 43)!!

    It is reading the fasta file as if it has 82 sequences, when it really has the same amount as the mRNA fasta file. I've never had this problem before, what am I missing? Thank you for the help . Attached are both files.

    https://www.dropbox.com/s/z9k0pkr5g1...fFT.fasta?dl=0 Protein alignment

    https://www.dropbox.com/s/jpgdz213oa...FFT.fasta?dl=0 mRNA file

Latest Articles

Collapse

  • seqadmin
    Multiomics Techniques Advancing Disease Research
    by seqadmin


    New and advanced multiomics tools and technologies have opened new avenues of research and markedly enhanced various disciplines such as disease research and precision medicine1. The practice of merging diverse data from various ‘omes increasingly provides a more holistic understanding of biological systems. As Maddison Masaeli, Co-Founder and CEO at Deepcell, aptly noted, “You can't explain biology in its complex form with one modality.”

    A major leap in the field has
    ...
    02-08-2024, 06:33 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 08:52 AM
0 responses
21 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-20-2024, 08:57 AM
0 responses
13 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-14-2024, 09:19 AM
0 responses
49 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-12-2024, 03:37 PM
0 responses
435 views
0 likes
Last Post seqadmin  
Working...
X