Can some body suggest me how to compare two DNA sequences.Please note that the sequences which i have are in ABI format, Actually i have tried in blastn but it hasn't accepted in the above file format.Hence i have converted the file to fasta.but still i am unable to..can some one help me out in this...
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
If your basecalling/conversion was succesful you should have something that looks like this in a text editor.
Try one of these tools: http://www.ebi.ac.uk/Tools/psa/ or BLAST two sequences
-
This program (http://www.dnabaser.com/download/chromatogram-explorer/) can read ABI files in and compare them directly. There are many commercial programs that can read ABI files but you probably don't have access to one.
If you have managed to convert ABI files to fasta format then sarvidsson already has provided some suggestions. You could also simply use clustalX to do a pair-wise sequence alignment (if the two sequences are similar).
Comment
Latest Articles
Collapse
-
by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
Channel: Articles
Yesterday, 07:01 AM -
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 04-11-2024, 12:08 PM
|
0 responses
59 views
0 likes
|
Last Post
by seqadmin
04-11-2024, 12:08 PM
|
||
Started by seqadmin, 04-10-2024, 10:19 PM
|
0 responses
57 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 10:19 PM
|
||
Started by seqadmin, 04-10-2024, 09:21 AM
|
0 responses
47 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 09:21 AM
|
||
Started by seqadmin, 04-04-2024, 09:00 AM
|
0 responses
55 views
0 likes
|
Last Post
by seqadmin
04-04-2024, 09:00 AM
|
Comment