Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Trimmomatic command not found error

    Hi there,

    I am trying to run Trimmomatic-0.33 to trim illumina adaptors from paired end reads.

    The command I am running is :

    Code:
    java -jar trimmomatic-0.33.jar PE -phred33 reads1.fastq reads2.fastq paired1.fastq unpaired1.fastq paired2.fastq unpaired2.fastq ILLUMINACLIP:Truseq_PE.fa:2:30:10:1:true
    But I am getting the following error message:

    Code:
    /usr/bin/java: line 1: PK: command not found
    /usr/bin/java: line 2:?x: command not found
    /usr/bin/java: line 3: syntax error near unexpected token `???D????{?T?.??ת?9??PK'
    /usr/bin/java: line 3:?x>F??\??META-INF/MANIFEST.MFM?=? w???GSS?V;7.ƕ?ҧ?h??{????F??N???R<	???>??~A?$??-U?ِ	+???????i?}M??i@?F???;?????zC?K?X]?L?=g#?(???D????{?T?.??ת?9??PK'
    I would be grateful for any assistance in interpreting this error message,
    thankyou.

Latest Articles

Collapse

  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
28 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
31 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
27 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
52 views
0 likes
Last Post seqadmin  
Working...
X