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  • charlieali
    Junior Member
    • Feb 2015
    • 1

    #1

    Trimmomatic command not found error

    Hi there,

    I am trying to run Trimmomatic-0.33 to trim illumina adaptors from paired end reads.

    The command I am running is :

    Code:
    java -jar trimmomatic-0.33.jar PE -phred33 reads1.fastq reads2.fastq paired1.fastq unpaired1.fastq paired2.fastq unpaired2.fastq ILLUMINACLIP:Truseq_PE.fa:2:30:10:1:true
    But I am getting the following error message:

    Code:
    /usr/bin/java: line 1: PK: command not found
    /usr/bin/java: line 2:?x: command not found
    /usr/bin/java: line 3: syntax error near unexpected token `???D????{?T?.??ת?9??PK'
    /usr/bin/java: line 3:?x>F??\??META-INF/MANIFEST.MFM?=? w???GSS?V;7.ƕ?ҧ?h??{????F??N???R<	???>??~A?$??-U?ِ	+???????i?}M??i@?F???;?????zC?K?X]?L?=g#?(???D????{?T?.??ת?9??PK'
    I would be grateful for any assistance in interpreting this error message,
    thankyou.

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